1st ESSIB, 2014 - University of Milano-Bicocca
Stability, Folding, and Misfolding of Recombinant Proteins
Oct 6-10, 2014
The 1st ESSIB took place 6. - 10. October 2014 at the University of Milano-Bicocca with the topic "Stability, Folding, and Misfolding of Recombinant Proteins".The summer school consisted of theory talks by 4 lecturers from Milano and 5 lecturers from Germany, Switzerland, Spain, Sweden, and Slowenia, three practical afternoon courses offered by the local organizers, and an excursion to the company DiaSorin. 22 PhD students and postdocs from European countries, Brazil and Armenia participated in the course.
Participants
- Hovsep Aganyants, National Academy of Sciences of the Republic of Armenia
- Celeste Annovazzi, CNR, Milano
- Ewelina Basiak, Warsaw University of Life Science and University in Burgundy
- Cinzia Bernardi, DIMS, Genova
- Mariangela Cerreta, CNR, Naples
- Lorena Cintra, University of Brasília
- Gianluca Conti, Insubria
- Federica Donnarumma, University of Naples
- Joseline Houwman, Wageningen, NL
- Mieke Huijbers, Wageningen, NL, group van Berkel
- Virginia Lorenzo, Caserta
- Carlotta Marchesi, CNR, Milano
- Fabiola Mascanzoni, CNR, Naples
- Elisa Mazzoleni, Diasorin, Gerenzano
- Dimitra Papaefthimiou, Aristotle University of Thessaloniki
- Francesco Piacente, University of Genova
- Stefania Enza Sestito, UNIMIB
- Giovanna Sociali, University of Genova
- Laura Strippoli, Diasorin, Gerenzano
- ... and 3 other participants from Brasil, France and Italy
Monday
| Opening and Lessons |
| 14.30 - 15.00: | Welcome and Introduction (Marina Lotti, Jürgen Pleiss) |
| 15.00 - 16.00: | Pierre Goloubinoff (Lausanne, CH) - Protein folding, misfolding and aggregation |
| 16.00 - 16.30: | Coffee |
| 16.30 - 17.30: | Presentation by the students (1st group) |
| 17.45 - 18.30: | Paola Branduardi (UNIMIB) - Synthetic biology and tools for recombinant proteins production |
| 19.00: | Welcome cocktail (Campus Bicocca, Harry's Bar) |
| |
Tuesday
| Lessons |
| 8.30 - 9.15: | Ario de Marco (Nova Gorica, SI) - Aggregates from recombinant proteins: preventing and reverting the aggregation process |
| 9.15 - 10.00: | Presentation by the students (2nd) group |
| 10.00 - 10.30: | Coffee |
| 10.30 - 11.15: | Silvia Maria Doglia (UNIMIB) - FT-IR spectroscopy to study protein aggregation in vitro and in vivo |
| 11.15 - 12.00: | Silvia Maria Doglia, Antonino Natalello (UNIMIB) - Introduction to FT-IR practicum |
| 12.00 - 13.00: | Lunch |
| Open Seminar |
| 13.30 - 14.30 | Ario de Marco (Nova Gorica, SI) - Recombinant antibody isolation, engineering into immune-reagents, and cost-effective production |
| Practicum |
| 14.30 - 16.30: |
| Group a | Silvia Maria Doglia, Antonino Natalello - Infrared spectroscopy |
| Group b | Paola Branduardi, Francesca Martani - Synthetic biology I |
| 16.30 - 17.00: | Coffee |
| 17.00 - 19.00: |
| Group a | Paola Branduardi, Francesca Martani - Synthetic biology I |
| Group b | Silvia Maria Doglia, Antonino Natalello - Infrared spectroscopy |
Wednesday
| Lessons |
| 8.30 - 9.15: | Ario de Marco (Nova Gorica, SI) - Identification and characterization of recombinant protein aggregates as part of the yield optimization procedure |
| 9.15 - 10.00: | Presentation by the students (3rd) group |
| 10.00 - 10.30: | Coffee |
| 10.30 - 11.30: | Rita Grandori (UNIMIB) - Unfolding and aggregation of proteins studied by mass spectrometry |
| Open Seminar |
| 11.30 - 12.30: | Salvador Ventura (Barcelona, E) - An integrated computational experimental approach to understand protein aggregation |
| 13.00 - 14.00: | Lunch |
| Lesson |
| 14.00 - 14.30: | Rita Grandori, Carlo Santambrogio (UNIMIB) - Introduction to mass spectrometry practicum |
| Practicum |
| 14.30 - 16.30: |
| Group a | Rita Grandori, Carlo Santambrogio - Mass spectroscopy |
| Group b | Paola Branduardi, Francesca Martani - Synthetic biology II |
| 16.30 - 17.00 | Coffee |
| 17.00 - 19.00: |
| Group a | Paola Branduardi, Francesca Martani - Synthetic biology II |
| Group b | Rita Grandori, Carlo Santambrogio - Mass spectroscopy |
Thursday
| Lessons |
| 8.30 - 9.30: | Salvador Ventura (Barcelona, E) - Prediction of aggregation propensity in protein sequences and structures |
| 9.30 - 10.30: | Andrea Dal Corso (Diasorin, I) - Bioreagents Development for in vitro diagnostics application. A quick tour on recombinant proteins production |
| 10.30 - 11.00: | Coffee |
| Open Seminar |
| 11.00 - 12.00: | Andreas Barth (Stockholm, Se) - Protein reactions studied by infrared spectroscopy |
| 12.00 - 13.00: | Lunch |
| Lesson |
| 13.00 - 14.00: | Carlo Santambrogio, Rita Grandori - Data processing and discussion of experimental results |
| Excursion to DiaSorin Research Center (Gerenzano) |
| 14.00 - 19.00: | Visit of company plant and labs |
Friday
| Lesson |
| 8.30 - 9.15: | Martin Siemann Herzberg (Stuttgart, DE) - Cell-free protein synthesis |
| Practicum |
| 9.30 - 10.30: | Salvador Ventura - Sequence analysis |
| 10.30 - 11.00: | Coffee |
| 11.00 - 13.00: | Salvador Ventura - Sequence analysis |
| 13.00 - 14.00: | Lunch |
| Closing |
| 14.00 - 14.30: | Closing remarks and certificates |
Marina Lotti
Paola Branduardi
Department of Biotechnology and Biosciences
University of Milano-Bicocca
Buildings U3-U4
Piazza della Scienza 2-4
20126 Milano
Italy
How to reach us
Please have a look on the map
The Department of Biotechnology and Biosciences is located in Building U3
There are several connection by public transport:
Metro 5 (Lilla) stop Bicocca. 1 stop from the hotel
Tram 7
Bus 87
Accommodation
Accomodation was provided in double rooms at:
Hotel IBIS Ca’ Granda, Viale Suzzani 13/15, 20162 Milano (Metro 5, Ca’ Granda)
